1. 计算pihat > 0.2的组合

plink --bfile HapMap_3_r3_10 --genome --min 0.2 --out pihat_min0.2

说明文档：

--genome invokes an IBS/IBD computation, and then writes a report with the following fields to plink.genome:

FID1	Family ID for first sample
IID1	Individual ID for first sample
FID2	Family ID for second sample
IID2	Individual ID for second sample
RT	Relationship type inferred from .fam/.ped file
EZ	IBD sharing expected value, based on just .fam/.ped relationship
Z0	P(IBD=0)
Z1	P(IBD=1)
Z2	P(IBD=2)
PI_HAT	Proportion IBD, i.e. P(IBD=2) + 0.5*P(IBD=1)
PHE	Pairwise phenotypic code (1, 0, -1 = AA, AU, and UU pairs, respectively)
DST	IBS distance, i.e. (IBS2 + 0.5*IBS1) / (IBS0 + IBS1 + IBS2)
PPC	IBS binomial test
RATIO	HETHET : IBS0 SNP ratio (expected value 2)

2. 提取Z1大于0.9的个体

awk '{if($8>0.9) print $0}' pihat_min0.2.genome  > zoom_pihat.genome

过滤出91个组合：

3. 作图

R代码

pdf("relatedness.pdf")
relatedness = read.table("pihat_min0.2.genome", header=T)
par(pch=16, cex=1)
with(relatedness,plot(Z0,Z1, xlim=c(0,1), ylim=c(0,1), type="n"))
with(subset(relatedness,RT=="PO") , points(Z0,Z1,col=4))
with(subset(relatedness,RT=="UN") , points(Z0,Z1,col=3))
legend(1,1, xjust=1, yjust=1, legend=levels(relatedness$RT), pch=16, col=c(4,3))

pdf("zoom_relatedness.pdf")
relatedness_zoom = read.table("zoom_pihat.genome", header=T)
par(pch=16, cex=1)
with(relatedness_zoom,plot(Z0,Z1, xlim=c(0,0.02), ylim=c(0.98,1), type="n"))
with(subset(relatedness_zoom,RT=="PO") , points(Z0,Z1,col=4))
with(subset(relatedness_zoom,RT=="UN") , points(Z0,Z1,col=3))
legend(0.02,1, xjust=1, yjust=1, legend=levels(relatedness$RT), pch=16, col=c(4,3))

pdf("hist_relatedness.pdf")
relatedness = read.table("pihat_min0.2.genome", header=T)
hist(relatedness[,10],main="Histogram relatedness", xlab= "Pihat")
dev.off()

这里的PO为：亲子关系 这里的UN为：非亲缘关系

4. 删除亲子关系的个体

plink --bfile HapMap_3_r3_10 --filter-founders --make-bed --out HapMap_3_r3_11
 

日志：

PLINK v1.90b6.5 64-bit (13 Sep 2018)           www.cog-genomics.org/plink/1.9/
(C) 2005-2018 Shaun Purcell, Christopher Chang   GNU General Public License v3
Logging to HapMap_3_r3_11.log.
Options in effect:
 --bfile HapMap_3_r3_10
 --filter-founders
 --make-bed
 --out HapMap_3_r3_11

515185 MB RAM detected; reserving 257592 MB for main workspace.
1073743 variants loaded from .bim file.
161 people (77 males, 84 females) loaded from .fam.
110 phenotype values loaded from .fam.
51 people removed due to founder status (--filter-founders).
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 110 founders and 0 nonfounders present.
Calculating allele frequencies... done.
Total genotyping rate in remaining samples is 0.998016.
1073743 variants and 110 people pass filters and QC.
Among remaining phenotypes, 55 are cases and 55 are controls.
--make-bed to HapMap_3_r3_11.bed + HapMap_3_r3_11.bim + HapMap_3_r3_11.fam ...
done.
 

可以看出，51个个体被移除。

5. 结果文件

HapMap_3_r3_11.bed  HapMap_3_r3_11.bim  HapMap_3_r3_11.fam  HapMap_3_r3_11.log

6. 注意

这里讲亲子关系的个体移除，不是必须要的，比如我们分析的群体里面有亲子关系的个体，想要进行分析，不需要做这一步的筛选。